FlowSOM offers a new way to look at your cytometry data. Instead of visualizing all the cells in 2D-scatter plots, the cells are first clustered. By grouping very similar cells together, it is easier to create a full overview, in which each group of cells is visualized as a 'node'. These nodes are organized into a tree, in which similar nodes are connected, creating 'branches' corresponding to the different cell types. Pie charts indicate the MFI's for different markers and node size corresponds to the number of cells in each population.