Background: Third generation sequencing platforms produce longer reads with higher error rates than second generation technologies. While the improved read length can provide useful information for downstream analysis, underlying algorithms are challenged by the high error rate. Error correction methods in which accurate short reads are used to correct noisy long reads appear to be attractive to generate high-quality long reads. Methods that align short reads to long reads do not optimally use the information contained in the second generation data, and suffer from large runtimes.
NXTGNT, the Ghent University reference center for next generation sequencing, offers a range of Illumina next generation sequencing services and support to the academic community and its external customers.
Determines the whole DNA sequence at high resolution and allows for screening of mutations, copy number variations, inserts, etc.
The study of heritable changes in gene function that occur without a change in the sequence of the DNA.