Tips for RT-qPCR gene expression of difficult to analyze paraffin-embedded tissue samples

Fragmented RNA from formalin-fixed paraffin-embedded (FFPE) tissue is a known obstacle to gene expression analysis. In this study, the impact of RNA integrity, gene-specific reverse transcription and targeted cDNA preamplification was quantified in terms of reverse transcription polymerase chain reaction (RT-qPCR) sensitivity by measuring 48 protein coding genes on eight duplicate cultured cancer cell pellet FFPE samples and twenty cancer tissue FFPE samples.

Nov/17 09:00 - Nov/18 18:00

2-day qPCR course Ghent

While the practical performance of a real-time PCR quantification experiment is relatively straightforward, it is clear that many users experience a genuine need for more in depth training of their experiment design and data-analysis skills.

Furthermore, it is usually hard to establish a consensus on how to perform a good qPCR experiment within a molecular laboratory team.

To accommodate this need, Biogazelle organizes focused courses in which the fundamental and advanced principles of experiment design, sample and assay quality control and data-analysis are covered.

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