Proteomics in the genome engineering era

Genome engineering experiments used to be lengthy, inefficient and often expensive, preventing a widespread adoption of such experiments for the full assessment of endogenous protein functions. With the revolutionary CRISPR/Cas9 technology, genome engineering became accessible to the broad life sciences community and is now implemented in several research areas. One particular field that can benefit significantly from this evolution is proteomics where a substantial impact on experimental design and general proteome biology can be expected.

Nov/26 11:00 - Nov/26 12:00
J Schell seminar room UGent-VIB Research Building FSVM Technologiepark 927 9052 Zwijnaarde (Gent)

BIG N2N seminar by Maarten Dhaenens: Data analysis in quantitative proteomics: framing a fuzzy picture of mass spectrometry

Commercial software tools are sold with a strong emphasis on user-friendliness, i.e. simplicity, giving rise to the impression that proteomics data is well understood. Yet the ion path that peptides travel through a mass spectrometer (an ESI-QTOF in this lecture) immediately provokes thought on just how a computer algorithm can cope with the complexity of the physical reality, which is reflected in the recorded data. Proteomics data still is over 50% of uninterpretable signals, with the other 50% prone to a lot of misinterpretation both at the level of identification and quantification.


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