Phospho-iTRAQ: Assessing Isobaric Labels for the Large-Scale Study Of Phosphopeptide Stoichiometry

The ability to distinguish between phosphopeptides of high and low stoichiometry is essential to discover the true extent of protein phosphorylation. We here extend the strategy whereby a peptide sample is briefly split in two identical parts and differentially labeled preceding the phosphatase treatment of one part. Our use of isobaric tags for relative and absolute quantitation (iTRAQ) marks the first time that isobaric tags have been applied for the large-scale analysis of phosphopeptides.

Jun/15 09:00 - Jun/16 18:00
Gent, Belgium

Next-Generation Antibodies and Protein Analysis: Tools and Technologies (VIB Conference)

VIB’s Tools and Technologies meetings provide a forum for top academic speakers and scientists from both established and emerging companies to present their latest scientific achievements and exciting technological solutions. The Next-Generation Antibodies and Protein Analysis conference will look at emerging tools and approaches in the fields of Proteomics, Single-Cell Proteome Analysis, Protein Modifications, Single Domain and Recombinant Antibodies, Affinity Reagents, Biosensing, Interactomics and Digital/Single-molecule Immuno-assays.


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