Affinity purification-mass spectrometry is one of the most common techniques for the analysis of protein-protein interactions, but inferring bona fide interactions from the resulting data sets remains notoriously difficult. We introduce SFINX, a Straightforward Filtering INdeX that identifies true-positive protein interactions in a fast, user-friendly, and highly accurate way. SFINX outperforms alternative techniques on two benchmark data sets and is available via the Web interface at http://sfinx.ugent.be/.
Titeca, Kevin; Meysman, Pieter; Gevaert, Kris; Tavernier, Jan; Laukens, Kris; Martens, Lennart; Eyckerman, Sven. SFINX: Straightforward Filtering Index for Affinity Purification Mass Spectrometry Data Analysis. JOURNAL OF PROTEOME RESEARCH, 15 (1):332-338 doi: 10.1021/acs.jproteome.5b00666