Entire qPCR workflow covered in two days.
Specialist course in the Doctoral School of Life Sciences and Medicine, organised by Biogazelle.
While the practical performance of a real-time PCR quantification experiment is relatively straightforward, it is clear that many users experience a genuine need for more in depth training of their experiment design and data-analysis skills.
Furthermore, it is usually hard to establish a consensus on how to perform a good qPCR experiment within a molecular laboratory team.
To accommodate this need, Biogazelle organizes focused courses in which the fundamental and advanced principles of experiment design, sample and assay quality control and data-analysis are covered.
- balanced mix between theoretical background and practical hands-on data-analysis
- complemented with training on Biogazelle’s own qbase+ software
- entire qPCR workflow is covered with a focus on experiment design and data-analysis
- attention for validation and quality control
- focus on gene expression (including microRNA)
Several courses are scheduled every year, check out the calendar. We also organize on-site courses in which your own case studies can be used as an example, please contact us if you're interested.
Targeted towards qPCR users with at least some basic level of experience. These courses are intended for all lab members dealing with qPCR; from lab technicians to lab managers. The aim is to bring everyone to the same level and to ensure high quality qPCR results at all times.
Five good reasons to come our course
- Throughout the course the trainers explain how quality control at each step in the qPCR workflow ensures accurate results.
- Normalization using a non-validated housekeeping gene may lead to errors more than 3-fold in 25% of the experiments. To avoid such errors, the students learn how to determine which and how many reference genes to use.
- Statistical analysis of qPCR results remains a bottleneck for many researchers. The course covers descriptive statistics to summarize qPCR results; moreover some key guidelines are provided to facilitate the selection of the most appropriate statistical test.
- Biogazelle’s qPCR course allows the students to master the MIQE guidelines and publish their qPCR results accordingly.
For course content and registration, see: