The Unipept web application supports biodiversity analysis of large and complex metaproteome samples. Its underlying index structure is designed to quickly retrieve all occurrences of a tryptic peptide in UniProtKB records. Taxon-specificity of the tryptic peptide is successively derived from these occurrences using a novel lowest common ancestor approach that is robust against taxonomic misarrangements, misidentifications and inaccuracies. Not taking into account this identification noise would otherwise result in drastic loss of information. This application consists of two basic functionalities: a peptide-based taxonomic identification and a multi-peptide dynamic diversity treemap analysis.
The updated version (2015) includes the addition of interactive sunburst and treeview visualizations to the multipeptide analysis, the foundations of an application programming interface (API) and a command line interface, updated data sources, and the open-sourcing of the entire application under the MIT license.


Mesuere, Bart; Debyser, Griet; Aerts, Maarten; Devreese, Bart; Vandamme, Peter; Dawyndt, Peter. The Unipept metaproteomics analysis pipeline. PROTEOMICS, 15 (8):1437-1442; SI 10.1002/pmic.201400361 APR 2015
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